Houghton J. (Ed.) Helicobacter Species: Methods and Protocols

Humana Press, 2012. - 258 p. ISBN: 1627030042Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states. Over the last several decades, scientists have come to understand that the interplay between the bacteria, the host, and the environment all contribute to the clinical outcome of infection. In Helicobacter Species: Methods and Protocols, expert researchers in the field detail many of the methods and which are now commonly used to study Helicobacter infection. These include protocols and methods that have evolved over time, and standards across the field have been established which are essential for optimal outcomes and to allow comparison of data across different laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.Helicobacter pylori: An Overview
Perspectives on Methodology for in vitro Culture of Helicobacter pylori
Successful Culture Techniques for Helicobacter Species : General Culture Techniques for Helicobacter pylori
Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures From Infected Rodents
Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis
The Helicobacter pylori cag Pathogenicity Island
Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori
A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori
Cell Culture Based Assays to Test for Bacterial Adherence and Internalization
Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence
Rodent Models of Helicobacter Infection, Inflammation and Disease
Bacterial Culture and Inoculation of Mice (Simple Infection)
Adoptive Transfer of Splenocytes to Immunocompromised Mice
Isolation of Gastric Lamina Propria Leukocytes (LPL)
Delayed-type Hypersensitivity Determination
Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression
Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse
Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice
Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes
Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections
Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models
Innate Immune Responses to Helicobacter pylori Infection- An Overview
Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection
Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection
In vivo Measurement of Helicobacter pylori Infection